Do you know genetic excitant?

Do you know genetic excitant?
The intersection of kingdom and the intersection of university and cell and the intersection of molecule and the intersection of nerve and scientific the intersection of department and so as to use gene to be excited in athlete research results that DJWells obtain in human gene therapy, London of Britain, pharmaceutical become perhaps, in addition present detection method unripe, therefore sports genetic use of excitant might become general in the competitive sport in recent years. Therefore, improving and improvement of the detection method that is controlled technically seems extremely important. Operate the result of study that can improve muscle performance with the heredity of several laboratory rodents to issue, athletes used the gene to strengthen the possibility that pharmaceuticals are usually said genetic excitant to arouse the attention from people. With other therapeutic method ' Such as taking serious histone) Compare, an outstanding benefit of gene therapy is, the internal albumen can be produced continuously, and avoided looking like other injectable preparation and problem of drug effect dynamics with peak and valley in the body of one kind of medicines of similar injectable preparation. Genetic excitant is applied to the clinical trial only on animal at present, though gene therapy has already made some prominent success, most treatments of disease of this domain mankind are still at animal's model level. Because the little mouse individual is little, the vitality is strong, can obtain the ideal result with the high carrier dosage, use for human body could win the same result, yet have no answer with appropriate carrier, acceptable dosage. For example, the effective dose of AAV while sending in the little mouse systematically is 4* 1012 carrier particles. In order to apply to the adult human body, needs 4* 1015 carrier particles, but this dosage has already exceeded the production capacity of most research and commerce at present. There is even the extremely individual laboratory, can only produce and deal with enough virus that a dog used of one sharp. By contrast, the plasmid DNA is easier to control than a large amount of easy purity for producing and the products. But the plasmid transgenics efficiency is relatively low, someone tries to be improving through the physical method to send, such as from circulating the limbs separated temporarily to irritate and flow wholly, belong to the relative " low scientific and technological content " with tourniquet Method,last laboratories illegal. But success of this kind of method on the human body has no report. The production of the for another example widowed nucleotide needs to prevent enzyme from degrading, because of the patent question, only limited to few suppliers at present, therefore narrow the athlete's possibility to use these operational reagent of heredity that can't be measured. Apply to human body's causing the risk that tumour or death is involved in genetic excitant mainly in two main fields. It is product and procedure that gene send that have risk at first, gene controlled to it expresses oneself to be harmful secondly. Adenovirus carriers very correlate with mortality in some human gene therapy tests clearly, died in the same way after the blood vessel treatment in 1999. Growth hormone and insulin kinds of 1 growth factor two urging the intersection of cell division and pharmaceutical and resisting, withering, losing pharmaceuticalling effective, can lead to the fact danger increased happens, a lot of research has expounded this kind of risk already in tumour. Similar to this, the hypoxia may cause the function of better blood vessel while producing crossing the expression of the factor while inducing factor 1 and blood vessel, thus promote the development of entity tumour and encourage the growth of tumour. Erythropoietin (Epo) If you can't pass by expression, there will be security risk that is potential. Epo treatment can cause the increase of hematocrit, thus make the blood stickier, increase the heart to bear. This kind of consequence may include circulating and blocking a little, apoplexy and heart are depleted. In addition, the production of Epo has already caused anaemia of one's own immunity in the macaque after the transgenics. Just as bringing out and what natural muscle growth inhibin is seen while striking and getting rid of the little mouse, totally blocking the reducing that can cause the muscular strength of the quality ratio of inhibin activity of growth of muscle, muscle though the intersection of growth and the intersection of inhibin and the intersection of defect and little mouse have old muscle, strength but than getting wild with little mouse loud. Whether athletes use and can't detect the systematic expression albumen such as Epo and small molecule inducing peculiar heredity to change to whole body yet temporarily, it is riper to control the method, and can offer the indirect evidence for possible genetic excitant. Other heredity is very difficult to be measured to to decorate. It is apter to resume after muscles are loose and wounded that muscles are peculiar to express leading to the fact as IGF-1 montages and makes a variation the body, but does not cause the increase of IGf-1 level in the blood. And the detection of excitant depends on urine and blood sample, and usually think that it is unacceptable to organize examining alive at present. Even if use muscles to live and examine while suspecting the athletes who use genetic excitant in those strongly, samples fetched still exist can't point out the whole muscle, the position is not that a gene sends issues such as 100% of the effective positions,etc.. Therefore the possibility of this kind of detection is limited, and face the severe technological challenge. The future method can detect the change of gene expression especially in the blood leucocyte ' Duplicate the group to study) China, the change (protein group studies) that the protein expresses in the blood or urine ,In the and the blood or urine the change of metabolite ' Group studies the supersession) . Direct challenge of method these lie in, define what level a normal one and what change may offer the undoubted excitant evidence. Because individual hereditary table, diet and other environmental factors, actually have a lot of parameters. If only because its endogenous hereditary table picture demonstrates the phenomenon more normal and more competent than some peculiar genetic products and suspends it, that is unfair. In this way, the modes of mRNA, protein or metabolite can not be used for measuring when being changed, and need to gather every athlete's long-term sample and deciding their normal base-line value, therefore will initiate a very outstanding coordination task and extra cost, very obvious this is a problem needing to further study and discuss at the international level. In a word, the prospect of genetic excitant is basically the aspect of theory at present. However, the whole field should continue controlling closely, some athletes are probably seduced into accepting to test in spite of the unknown risk after all.

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